IgG Platelet Antibodies in EDTA-Dependent Pseudothrombocytopenia Bind to Platelet Membrane Glycoprotein IIb
نویسندگان
چکیده
منابع مشابه
Temperature-dependent effects of EDTA on the membrane glycoprotein IIb-IIIa complex and platelet aggregability.
In agreement with previous studies, we observed that incubation of washed human platelets with EDTA at 37 degrees C for short periods caused an irreversible loss of their aggregation response to adenosine diphosphate and markedly diminished their capacity to bind fibrinogen. AP-2 is a monoclonal antibody that reacts with a determinant specific to the glycoprotein (GP) IIb-IIIa complex. We now r...
متن کاملThe platelet membrane glycoprotein IIb-IIIa complex.
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متن کاملEDTA dependent pseudothrombocytopenia caused by antibodies against the cytoadhesive receptor of platelet gpIIB-IIIA.
AIMS To clarify the mechanisms involved in the development of EDTA dependent pseudothrombocytopenia, particularly the platelet receptors. METHODS Platelets were measured in 33 patients with pseudothrombocytopenia, using different anticoagulants to collect blood samples (direct test). The results were compared with the counts obtained by adding patients' serum or immunoglobulins to normal bloo...
متن کاملMonoclonal antibodies to human platelet glycoprotein IIb beta that initiate distinct platelet responses.
Hybridomas secreting monoclonal antibodies (MoAbs) to human platelet membrane glycoprotein IIb (GPIIb) were prepared by fusing cells of a mouse myeloma line to spleen cells from a BALB/c mouse immunized with purified GPIIb. Six of the hybridomas secreted MoAbs that recognized epitopes on the 23,000-dalton, disulfide-linked subunit of GPIIb, GPIIb beta. All six of these MoAbs agglutinated platel...
متن کاملPlatelet Glycoprotein IIb/IIIa Inhibitors
Glycoprotein IIb/IIIa (GPIIb-IIIa) complexes (integrin aIIbb3) mediate platelet aggregation by binding fibrinogen or von Willebrand factor (vWF), protein cofactors that form bridges between adjacent platelets. The cross-linked adhesive proteins assemble platelets into the aggregate. Agents that block the function of the GPIIb-IIIa complex of platelets constitute a powerful new generation of ant...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 1998
ISSN: 0002-9173,1943-7722
DOI: 10.1093/ajcp/110.2.178